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当前位置: 首页 > 产品中心 > Anti_chicken > Iwai-Chem/Chicken IFN-Alpha(Interferon Alpha) ELISA Kit/ELISA Kit/E-EL-Ch0130
商品详细Iwai-Chem/Chicken IFN-Alpha(Interferon Alpha) ELISA Kit/ELISA Kit/E-EL-Ch0130
Iwai-Chem/Chicken IFN-Alpha(Interferon Alpha) ELISA Kit/ELISA Kit/E-EL-Ch0130
Iwai-Chem/Chicken IFN-Alpha(Interferon Alpha) ELISA Kit/ELISA Kit/E-EL-Ch0130
商品编号: E-EL-Ch0130
品牌: Iwai
市场价: ¥11200.00
美元价: 6720.00
产地: 美国(厂家直采)
公司:
产品分类: 抗鸡
公司分类: Anti_chicken
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description Enzyme Immunoassay for the determination of Chicken IFN-α.
Method Type Sandwich-Ab
Sensitivity 9.38 pg/mL
Assay Range 15.63~1000 pg/mL
Other Name IFL, IFNA
Principle This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Chicken IFN-α. Standards or samples are added to appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibodies specific for Chicken IFN-α and Avidin- Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. After incubation, free components are washed away. Then the Substrate Reagent is added to each well, only those wells that contain Chicken IFN-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme- substrate reaction will be terminated by adding Stop Solution and appears yellow in color. The optical density (OD) can be measured with spectrophotometry at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Chicken IFN-α. The concentration of Chicken IFN-α in samples can be calculated by comparing the OD of the samples with the standard curve.
Intended Use This ELISA kit can be applied to the in vitro quantitative determination of Chicken IFN-α concentrations in serum, plasma and other biological fluids.
Specificity This kit recognizes some recombinant and natural Chicken IFN-α. No significant cross-reactivity or interference was observed.
Sample Type Serum, plasma and other biological fluids
Kit Components Micro ELISA Plate, 8 x 12 well strips
Reference Standard, 2 vials
Concentrated Biotinylated Detection Ab(100x), 120 µL
Concentrated HRP Conjugate(100x), 120 µL
Reference Standard and Sample Diluent, 20 mL
Biotinylated Detection Ab Diluent, 14 mL
HRP Conjugate Diluent, 14 mL
Concentrated Wash Buffer (25x), 30 mL
Substrate Reagent, 10 mL
Stop Solution, 10 mL
Plate Sealer, 5 pieces
Product Manual
Certificate of Analysis
Other supplies required Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37°C Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
Storage All reagents in this kit should be stored in accordance with the information on product labels. Unused wells should be returned to the foil pouch with the desiccant pack and resealed until air-tight. The Substrate Reagent should not be kept at -20°C. Avoid exposing reagents to strong light during storage or the incubation process. Caps of reagents should be kept tightly sealed to prevent evaporation and microbial contamination. Erroneous results may occur if reagents are not stored as advised above.

Data Sheet E-EL-Ch0130 (PDF File)


Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .

FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd

 

      Questions about this product?   Ready to place an order?
Email us at orders@iwai-chem.com or give us a call: (650) 486-1541

品牌介绍
Iwai-Chem岩井北美有限公司是岩井化学株式会社(位于日本)的美国全资子公司,成立于2006年,是岩井向国际市场扩张的一部分。致力于弥合美国和日本生命科学产业之间的鸿沟,北美洲岩井提供了从日本到美国市场的高质量试剂和研究工具,同时还向日本提供了美国技术和产品。    历史:* 1947年4月–岩井化学株式会社在日本东京成立 * 2006年10月–岩井北美有限公司在加利福尼亚州伯灵格姆成立。* 2007年9月–收购了Iwai USA Inc.及其所有资产,  包括Chondrex,Inc.的多数股权。 * 2009年2月–办公室迁至加利福尼亚福斯特城* 2017年4月-办公室搬迁至加利福尼亚州圣卡洛斯的当前地址 关于  岩井化学株式会社  (日本): 岩井化学株式会社成立于1947年,位于日本东京,旨在生产和分配高纯度试剂。在过去的七十年中,岩井化学一直秉持“诚信”作为其管理理念的基石,并深知只有赢得客户的信任,企业才能继续创新和发展。自岩井创立以来,生命科学研究领域取得了显着进展,特别是在分子生物学,基因工程和免疫学领域。通过对这些研究领域中对专用试剂的需求进行预测并采取行动,并与国内外主要制造商建立合作伙伴关系,从而形成了一条完整的产品线,岩井化学已达到了目前的行业领导者地位。如今,岩井化学公司拥有70多名销售人员,为450多家制药公司,大学和研究机构的2500多个研究实验室提供服务,年销售额约为1.6亿美元,这使岩井化学公司占据了日本全部生物制品的10%试剂零售市场。