Product Description
| Product Name | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| 4EBP1 Monoclonal Antibody | E-AB-27001 | 133 | IgG2b | WB,IHC,IF,ELISA* | Human,Mouse,Rat | 60μL, 120μL, 200μL |
Description
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1),is a member of 4EBPs family,which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E,thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors,hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1,alpha,beta and gamma.
Spec Sheet E-AB-27001 (PDF File)
| Synonyms | 4E BP1,4EBP1,BP 1,eIF4E binding protein 1,EIF4EBP1,PHAS I |
| Swiss-Prot | Q13541 |
| Concentration | 1 mg/mL |
| Host | Mouse |
| Immunogen | Fusion protein of 4EBP1 |
| Purification Method | Protein A purification |
| Formulation | PBS with 0.02% sodium azide, 50% glycerol, PH7.3 |
| Storage | Store at -20?. Avoid freeze / thaw cycles. |
| Dilution | WB 1:1000-1:10000, IHC 1:50-1:500, IF 1:20-1:200 |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by:Elabscience
